CLOSTAT™

New Zealand

CLOSTAT™ IN SWINE

Stressors Affect Gut Health and Intestinal Intergrity

The intestinal tract of healthy swine contains a great number of different microorganisms whose balance promotes digestion, absorption of nutrients and overall health of the intestine. These microorganisms are both beneficial bacteria, such as Lactobacillus and Bifidobacterium spp, and pathogenic bacteria, such as Escherichia coli, Salmonella and Clostridium spp. Under normal circumstances, there is equilibrium between the bene cial and the pathogenic bacteria called eubiosis.

Sows are subjected to many stressors during their reproductive life cycle of breeding, gestation, farrowing and lactation. During these phases, different housing, management and feeding conditions affect the intestine micro ora balance. The result may be decreased maternal performance, reproductive efficiency and increased morbidity and mortality of nursing piglets.

Comparison of Sow Parameters

An improved maternal line of sows housed in a typical production unit were fed Bacillus subtilis PB6 for 12 weeks. Production parameters on the farm were compared to the previous 12 week period where no Bacillus subtilis PB6 was fed:

Bacillus subtilis (PB6) fed to sows resulted in a 3 to 5 days improvement in non-productive sow days and a 0.36% reduction in pre-weaning mortality. Pigs nursing Bacillus subtilis PB6 sows were heavier (12.92# versus 12.84#) and there were more of them (9.75 versus 9.63) at weaning. Sow mortality also decreased from 9.52 to 9.05%.

A Sow Lactation Trial Conducted in a Typical Production Unit

CLOSTAT™ IN POULTRY

Manifestation of Clostridial Enteritis

Poultry producers see signs of specific growth retardation around the 23rd day of poultry age (Graph 1) or even earlier. Litter quality changes and becomes wetter, leading to increase moisture levels and more watery zone around the droppings (Figure 1).

Consequences of poor litter quality are increased foot pad lesions, hock lesions and breast blister resulting in higher levels of rejections at the processing plant.

CLOSTAT™ - The Active Probiotic Probiotic that Prevents Clostridial Proliferation

Prevention of Clostridial enteritis can be done by using CLOSTAT™. CLOSTAT™ contains a unique strain of Bacillus subtilis PB6 that was isolated from the gut of healthy chickens. CLOSTAT™ has a targeted effect against C. perfringens (Figure 2). Numerous research and field research trials have demonstrated that CLOSTAT™ is a reliable solution for Clostridial infections.

CLOSTAT™ Mode of Action

Function of Fengycin

It is hypothesized that fengycin outcompetes Auto Inducing Peptide (AIP), the quorum-sensing signal that initiates host colonization and toxin production by C. perfringens at high cell density. The binding of fengycin to the AgrC-like receptor inhibits colonization and toxin production by C. perfringens.

Function of Surfactin

The B. subtilis PB6 in CLOSTAT™ has been found to secrete one or more biocidal proteins that are inhibitory towards certain strains of pathogenic bacteria, such as Clostridium spp. These proteins disrupt the membrane of bacteria, causing leakage of the cell contents and ultimately killing the pathogenic bacteria without harming the beneficial gut microflora.

CLOSTAT™ Highlights

CLOSTAT™ is compatible with common feed additives and most coccidiostats used in the feed industry and is heat stable under normal commercial pelleting conditions.

Exceptional customer service with best in class technical, laboratory and customer support from our dedicated teams and well-equipped laboratory.

• Clostridium Test Kits
• Farm and facility audits to identify gut health issues
• Feed and premix analysis to ensure recovery of CLOSTAT™

Necrotic Enteritis Challange Trial

A 27-day experiment was conducted, all birds were challenged on day 14 with a mixed coccidial inoculum and afterwards they received an infection with C. perfringens to induce necrotic enteritis.

In the control group, the infection caused mortality up to 36% confirming the challenge stress exposed to the birds (Graph 2). Addition of B. subtilis PB6 at 1 x 108 CFU/kg of feed, reduced the mortality towards 9.4%, whereas BMD (50 g/MT) lowered the mortality towards 17.2%.

A 27-day experiment was conducted, all birds were challenged on day 14 with a mixed coccidial inoculum and afterwards they received an infection with C. perfringens to induce necrotic enteritis.

In the control group, the infection caused mortality up to 36% confirming the challenge stress exposed to the birds (Graph 2). Addition of B. subtilis PB6 at 1 x 108 CFU/kg of feed, reduced the mortality towards 9.4%, whereas BMD (50 g/MT) lowered the mortality towards 17.2%.

Commercial Trial in Asia

CLOSTAT™ trial in replacing AGP in a commercial broiler was conducted in Asia.